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KMID : 0361020000430111096
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Korean Journal of Otolaryngology - Head and Neck Surgery
2000 Volume.43 No. 11 p.1096 ~ p.1101
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The Effect of Clucocorticosteroids on Eosinophil Apoptosis Primed by CulturedNasal Polyp Epithelial Cell Supernatant
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Shin Seung-Heon
Ye Mi-Kyung
Choi Jae-Wook
Kim Sang-Gyung
Jeong Hyo-Soon
Park June-Sik
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Abstract
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Background and Objectives: Eosinophil infiltration into inflammatory site is a characteristic histological finding in patient allergic rhinitis, bronchial asthma, and nasal polyps. The regulation of eosinophil survival and apoptosis may play a major role in tissue eosinophilia, and glucocorticosteroids (GCs) have been used therapeutically for nasal polyps. The purpose of this study is to investigate the effect of commonly used GCs on eosinophil survival and apoptosis primed by nasal polyp epichelial cells.
Materials and Methods: Peripheral blood eosinophils were incubated in increasing concentrations (10-8-10-5M) to GCs (triamcinolone, dexamethasone, budesonide, and fluticasone propionate) from January, 1990 to December, 1999 prior to the addition of human nasal polyp epithelial cell conditioned media (HECM). Eosinophil viability was measured with a MTS assay and apoptosis analyzed with the caspase-3 staining.
Results: GCs suppressed the HECM induced prolongation of eosinophil survival apoptotic change of cells from 2 days after incubation. Fluticasone propionate showed the strongest effects and triamcinolone showed the weakest effects.
Conclusion: GCs may diminish eosinophilic infiltration into nasal polyp by decreasing eosinophil viability, and abrogate the promoting effect of nasal epithelial cells.
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KEYWORD
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Eosinophils, Glucocorticoids, Nasal polyps, Apoptosis
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